Article
Paternity exclusion in koalas using hypervariable microsatellites.
The Journal of heredity - 1 Jan 2000
Houlden B A, England P, Sherwin W B
Abstract excerpt
Koala microsatellite loci containing the dinucleotide motif (CA)n were isolated from a size-fractionated (250-500 bp) koala genomic library and sequenced. Six locus-specific primer pairs were designed and synthesized for DNA amplification using the polymerase chain reaction (PCR). Microsatellite genotyping of 12 individuals generated unique "fingerprints" for each koala. All six microsatellite loci were...
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