Article
Characterization of large CTG repeat expansions in myotonic dystrophy alleles using PCR.
Human mutation - 1 Jan 1996
Cheng S, Barceló J M, Korneluk R G
Abstract excerpt
The CTG trinucleotide repeat expansions that are associated with myotonic dystrophy can be up to several thousand repeat units in length. We have developed a PCR protocol that has the potential to amplify mutant alleles with very large numbers of CTG repeats. The amplification uses the rTth DNA p...
Topics
- Alleles
- Base Sequence
- Blotting, Southern
- DNA Primers
- Humans
- Molecular Sequence Data
- Myotonic Dystrophy
- Polymerase Chain Reaction
- Trinucleotide Repeats