Article
High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes.
Acta biologica Hungarica - 1 Jan 1995
Jafari M A, Kiss J, Gergácz J, Heszky L E
Abstract excerpt
In order to develop a simple and feasible approach to achieve high frequency plant regeneration for protoplast isolation and transformation experiments, a method was elaborated by using a new type of explant (petiole segments) for the four Populus nigra genotypes. Callus initiation from the petioles took place on N6 medium containing 2,4-D (0.1-1 mg/l). The highest rate of callus initiation (100%) was achieved...
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