Characteristics of Na+/K(+)-ATPase mediated proton current in Na(+)- and K(+)-free extracellular solutions. Indications for kinetic similarities between H+/K(+)-ATPase and Na+/K(+)-ATPase.

Abstract excerpt

The Na+/K(+)-ATPase of an ouabain-resistant mutant of Torpedo californica and of rat was expressed in Xenopus oocytes by microinjection of mRNA coding for the alpha- and the beta-subunit of the protein. Electrophysiological measurements were performed by means of the extracellular giant-patch clamp technique. The pump currents were analyzed in nominal absence of extracellular Na+ and K+ ions. Under these...

Topics

• Adenosine Triphosphate
• Animals
• Drug Resistance
• Electric Conductivity
• Female
• Gene Transfer Techniques
• H(+)-K(+)-Exchanging ATPase
• Hydrogen-Ion Concentration
• Mice
• Mutation
• Oocytes
• Ouabain
• Patch-Clamp Techniques
• Rats
• Recombinant Proteins
• Sodium-Potassium-Exchanging ATPase
• Torpedo
• Xenopus laevis