Article
Identification of domains in human beta-hexosaminidase that determine substrate specificity.
The Journal of biological chemistry - 19 Jul 1996
Pennybacker M, Liessem B, Moczall H, Tifft C J, Sandhoff K, Proia R L
Abstract excerpt
The lysosomal beta-hexosaminidases are dimers composed of alpha and beta subunits. beta-Hexosaminidase A (alphabeta) is a heterodimer, whereas hexosaminidase B (betabeta) and S (alphaalpha) are homodimers. Although containing a high degree of amino acid identity, each subunit expresses a unique a...
Topics
- Amino Acid Sequence
- Base Sequence
- Carbohydrate Sequence
- Cloning, Molecular
- DNA Primers
- G(M2) Ganglioside
- Genetic Variation
- HeLa Cells
- Hexosaminidase B
- Humans
- Isoenzymes
- Kinetics
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Recombinant Fusion Proteins
- Restriction Mapping
- Substrate Specificity