Use of the Polymerase Chain Reaction and DNA Sequencing for Detection of Bartonella quintana in the Aortic Valve of a Patient with Culture-Negative Infective Endocarditis

Abstract excerpt

We used the polymerase chain reaction (PCR) and broad-range bacterial primers, combined with DNA sequencing, to identify Bartonella quintana as the etiologic agent in a case of culture-negative infective endocarditis; all blood cultures, as well as the bacterial cultures of the resected aortic valve and vegetations, remained negative. PCR was used to amplify bacterial 16S rDNA from a template prepared from the...

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