Article
Flow sorting of the Y sex chromosome in the dioecious plant Melandrium album.
Cytometry - 1 Dec 1995
Veuskens J, Marie D, Brown S C, Jacobs M, Negrutiu I
Abstract excerpt
The preparation of stable chromosome suspensions and flow cytometric sorting of both the Y sex chromosome of the white campion, Melandrium album, and the deleted Y chromosome of an asexual mutant, 5K63, is described. The principle has been to maintain transformed roots in vitro, synchronise and block mitosis, reduce cells to protoplasts, and lyse these to release chromosomes. Such in vitro material, unlike many...
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