Article
IS1533-based PCR assay for identification of Leptospira interrogans sensu lato serovars.
Journal of clinical microbiology - 1 Dec 1995
Zuerner R L, Alt D, Bolin C A
Abstract excerpt
A PCR-based assay was developed for typing L. interrogans sensu lato serovars. The assay is designed to exploit the presence of many copies of the leptospiral insertion sequence IS1533 and IS1533-like sequences present in the genomes of most leptospiral serovars. The PCR primers were designed to amplify DNA of unknown sequence between closely placed IS1533 or IS1533-like sequences. Amplification reactions primed...
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