Article
Substitution of two histidine residues in YadA protein of Yersinia enterocolitica abrogates collagen binding, cell adherence and mouse virulence.
Molecular microbiology - 1 Jun 1995
Roggenkamp A, Neuberger H R, Flügel A, Schmoll T, Heesemann J
Abstract excerpt
The plasmid-encoded surface protein YadA of Yersinia enterocolitica mediates binding to diverse extracellular matrix (ECM) proteins, adherence to epithelial cell lines, resistance to complement lysis, autoagglutination, and is required for mouse virulence. Using site-directed mutagenesis we attem...
Topics
- Adhesins, Bacterial
- Animals
- Bacterial Adhesion
- Base Sequence
- Cell Division
- Cloning, Molecular
- Collagen
- DNA Primers
- Diethyl Pyrocarbonate
- Extracellular Matrix Proteins
- Fibronectins
- Histidine
- Humans
- Mice
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Point Mutation