Article
Simultaneous detection of fourteen Italian cystic fibrosis mutations in seven exons by reverse dot-blot analysis.
Molecular and cellular probes - 1 Oct 1995
Rady M, D'Alcamo E, Seia M, Iapichino L, Ferrari M, Russo S, Romeo G, Maggio A
Abstract excerpt
The increasing number of cystic fibrosis (CF) mutations is a great obstacle to the use of DNA procedures in the detection of gene defects. We describe a fast, cheap and non-radioactive procedure, Reverse dot-blot analysis (RDB), for the simultaneous detection of CF mutations in the Italian popula...
Topics
- Alleles
- Base Sequence
- Cystic Fibrosis
- DNA Primers
- Exons
- Humans
- Italy
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Point Mutation
- Polymerase Chain Reaction
- Sequence Deletion