Article
Newly synthesized protein(s) must associate with p34cdc2 to activate MAP kinase and MPF during progesterone-induced maturation of Xenopus oocytes.
The EMBO journal - 15 Nov 1995
Nebreda A R, Gannon J V, Hunt T
Abstract excerpt
The meiotic maturation of Xenopus oocytes triggered by progesterone requires new protein synthesis to activate both maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAP kinase). Injection of mRNA encoding mutant p34cdc2 (K33R) that can bind cyclins but lacks protein kinase...
Topics
- Animals
- Antibodies, Monoclonal
- CDC2 Protein Kinase
- Calcium-Calmodulin-Dependent Protein Kinases
- Cells, Cultured
- Cyclins
- Enzyme Activation
- Enzyme Inhibitors
- Glutathione Transferase
- Maturation-Promoting Factor
- Mutation
- Oogenesis
- Progesterone
- Protein Binding
- Protein Precursors
- Proto-Oncogene Proteins c-mos
- RNA, Messenger
- Recombinant Fusion Proteins