Thyroxine increases the levels of epidermal growth factor messenger ribonucleic acid (EGF mRNA) in the thyroid in vivo, as revealed by quantitative reverse transcription polymerase chain reaction with an internal control EGF mRNA.

Abstract excerpt

Treating mice with T4 increases the level of immunoreactive epidermal growth factor (EGF) in the thyroid. In order to establish whether this response might reflect a T4-dependent increase in the levels of messenger RNA (mRNA) in the thyroid, we prepared an internal standard which permits us to quantitate EGF mRNA levels by reverse transcription plus polymerase chain reaction amplification (RT-PCR). Our synthetic...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Gene Deletion