Article
[Specific proteolysis of Clostridium thermocellum endoglucanase upon heterologous expression in Escherichia coli cells].
Genetika - 1 Feb 1993
Aminov R I, Golovchenko N P, Singkh R N, Akimenko V K
Abstract excerpt
The cel(1) gene of Clostridium thermocellum encoding endoglucanase cloned earlier in Escherichia coli was deleted at specific sites and fused with the E. coli gene lacZ'. Analysis of heterologous expression of the produced variants was performed. It was established that the post-translational modification of the clostridial endoglucanase in E. coli involves a membrane-linked N-terminal cleavage of 6 kDa...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
