Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Biosynthesis of lipoic acid: characterization of the lipoic acid auxotrophs Escherichia coli W1485-lip2 and JRG33-lip9.

Biochemistry - 13 Apr 1993

Hayden M A, Huang I Y, Iliopoulos G, Orozco M, Ashley G W

Abstract excerpt

The abilities of the Escherichia coli lipoic acid auxotrophs W1485-lip2 and JRG33-lip9 to grow on succinate medium in the presence of octanoate, 8-mercaptooctanoate, or 6-mercaptooctanoate have been determined. Both organisms are mutated in lipA. Neither organism can use octanoate or 6-mercaptooctanoate for production of lipoate, but the lip2 allele can use 8-mercaptooctanoate. Chromosomal DNA from the auxotrophs...

Topics

Amino Acid Sequence
Base Sequence
Caprylates
DNA, Bacterial
Deoxyribonuclease HindIII
Escherichia coli
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Thioctic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 8466915
DOI: 10.1021/bi00065a033

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Biosynthesis of lipoic acid: characterization of the lipoic acid auxotrophs Escherichia coli W1485-lip2 and JRG33-lip9.PMID 8466915Read ↗

Lipoic acid metabolism in Escherichia coli: the lplA and lipB genes define redundant pathways for ligation of lipoyl groups to apoprotein.Semantic proximity Read ↗

Assembly of the covalent linkage between lipoic acid and its cognate enzymes.Semantic proximity Read ↗

Assembly of Lipoic Acid on Its Cognate Enzymes: an Extraordinary and Essential Biosynthetic PathwaySemantic proximity Read ↗

Lipoic acid metabolism in Escherichia coli: isolation of null mutants defective in lipoic acid biosynthesis, molecular cloning and characterization of the E. coli lip locus, and identification of the lipoylated protein of the glycine cleavage system.Semantic proximity Read ↗

Repurposing lipoic acid changes electron flow in two important metabolic pathways of Escherichia coli.Semantic proximity Read ↗

Strategies for generating and utilizing phospholipid synthesis mutants in Escherichia coli.Semantic proximity Read ↗

Chromosomal amplification of the Escherichia coli lipB region confers high-level resistance to selenolipoic acid.Semantic proximity Read ↗

Scavenging of cytosolic octanoic acid by mutant LplA lipoate ligases allows growth of Escherichia coli strains lacking the LipB octanoyltransferase of lipoic acid synthesis.Semantic proximity Read ↗

Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates.Semantic proximity Read ↗

Isolation and characterization of LIP5. A lipoate biosynthetic locus of Saccharomyces cerevisiae.Semantic proximity Read ↗

The lipid A biosynthesis deficiency of the Escherichia coli antibiotic-supersensitive mutant LH530 is suppressed by a novel locus, ORF195.Semantic proximity Read ↗

Isolation and characterization of Burkholderia cenocepacia mutants deficient in pyochelin production: pyochelin biosynthesis is sensitive to sulfur availability.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.