Article
Extreme resistance to potato virus X infection in plants expressing a modified component of the putative viral replicase.
The EMBO journal - 1 Feb 1993
Longstaff M, Brigneti G, Boccard F, Chapman S, Baulcombe D
Abstract excerpt
Three types of mutation were introduced into the sequence encoding the GDD motif of the putative replicase component of potato virus X (PVX). All three mutations rendered the viral genome completely noninfectious when inoculated into Nicotiana clevelandii or into protoplasts of Nicotiana tabacum (cv. Samsun NN). In order to test whether these negative mutations could inactivate the viral genome in trans, the...
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