Article
Order of bovine DRB3, DYA, and PRL determined by sperm typing.
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Jan 1993
van Eijk M J, Russ I, Lewin H A
Abstract excerpt
The order and recombination fractions (theta) between the bovine major histocompatibility complex DRB3, DYA, and prolactin (PRL) genes were determined by typing of 254 sperm from a triply heterozygous bull. A recently developed method, primer extension preamplification (PEP), was used to amplify the bovine sperm genome prior to amplification of specific loci by the polymerase chain reaction (PCR). At least 28...
Topics
- Animals
- Base Sequence
- Cattle
- Chromosome Mapping
- DNA, Single-Stranded
- Genes, MHC Class II
- Genotype
- HLA-DR Antigens
- HLA-DRB3 Chains
- Histocompatibility Testing
- Male
- Molecular Sequence Data
- Polymerase Chain Reaction
- Prolactin
- Spermatozoa
