Article
Ordering three DNA polymorphisms on human chromosome 3 by sperm typing.
Genomics - 1 Jul 1991
Goradia T M, Stanton V P, Cui X F, Aburatani H, Li H H, Lange K, Housman D E, Arnheim N
Abstract excerpt
Three loci on the short arm of human chromosome 3 were ordered by sperm typing to expand the limited genetic map of this region. Almost 300 individual sperm from a donor triply heterozygous at D3S2, D3S11, and D3S12 were amplified by PCR using primers flanking the polymorphic site at each locus. Primary PCR product was reamplified using allele-specific primers of different lengths, allowing the allelic state at...
Topics
- Alleles
- Base Sequence
- Chromosome Mapping
- Chromosomes, Human, Pair 3
- DNA
- Genetic Markers
- Heterozygote
- Humans
- Male
- Models, Genetic
- Molecular Sequence Data
- Polymerase Chain Reaction