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Physiology: Transplantation of frozen—thawed mouse primordial follicles

1 Aug 1993

Abstract excerpt

Primordial follicles were isolated from juvenile mouse ovaries and cryopreserved by slow freezing with dimethylsulphoxide as the cryoprotectant. After thawing, approximately 80% of the oocytes and 65% of the somatic cells excluded Trypan Blue dye, indicating that cell membranes were still intact. Frozen-thawed cells were suspended in plasma clots and transplanted to the ovarian bursas of host animals that had...

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PMID: 8408511
DOI: 10.1093/oxfordjournals.humrep.a138221

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