Article
Extra-ovarian production of mature viable mouse oocytes from frozen primary follicles
1 Sept 1990
Abstract excerpt
Summary. Isolated primary mouse follicles can be frozen successfully and thawed in the presence of 1·5 m-DMSO. Similar proportions of freshly collected and frozen–thawed primary follicles undergo folliculogenesis in the absence of other ovarian tissue. Some of the mature oocytes recovered from these follicles were fertilized in vitro and, after transfer to pseudopregnant recipients at the 2-cell stage, developed...
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