Article
Methods incorporating a polymerase chain reaction and restriction fragment length polymorphism and their use as a 'gold standard' in diagnosing Old World cutaneous leishmaniasis.
Diagnostic microbiology and infectious disease - 1 Oct 2011
Azmi Kifaya, Nasereddin Abedelmajeed, Ereqat Suheir, Schnur Lionel, Schonian Gabriele, Abdeen Ziad
Abstract excerpt
Three polymerase chain reaction (PCR) assays - kinetoplast DNA (kDNA) PCR, 7SL PCR, ITS1 PCR - were compared for their ability to detect leishmanial parasites in the skin tissue aspirates of 212 Palestinian suspect cases of cutaneous leishmaniasis (CL). The kDNA PCR was the most sensitive, detecting 156/170 (91.8%) of positive samples confirmed by a set 'gold standard' but of poor specificity in identifying the...
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