Article
Identification of NOX2 regions for normal biosynthesis of cytochrome b558 in phagocytes highlighting essential residues for p22phox binding.
The Biochemical journal - 15 Dec 2014
Beaumel Sylvain, Grunwald Didier, Fieschi Franck, Stasia Marie José
Abstract excerpt
Cytochrome b558, the redox core of the NADPH oxidase (NOX) complex in phagocytes, is composed of NOX2 and p22phox, the synthesis of which is intimately connected but not fully understood. We reproduced 10 rare X-minus chronic granulomatous disease (CGD) mutations of highly conserved residues in NOX1-NOX4, in X0-CGD PLB-985 cells in order to analyse their impact on the synthesis of cytochrome b558. According to...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Cell Line
- Cytochrome b Group
- Humans
- Membrane Glycoproteins
- Molecular Sequence Data
- Mutation
- NADPH Oxidase 2
- NADPH Oxidases
- Phagocytes
- Protein Binding
- Protein Interaction Domains and Motifs
- Sequence Homology, Amino Acid