Dissociation and electrophoretic separation of dextranase and dextranase inhibitor from a tightly bound enzyme-inhibitor complex of Streptococcus sobrinus.

doi:10.1002/elps.1150140196

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Article

Dissociation and electrophoretic separation of dextranase and dextranase inhibitor from a tightly bound enzyme-inhibitor complex of Streptococcus sobrinus.

Electrophoresis - 1 Jul 1993

Wellington J E, Shaw J M, Walker G J

Abstract excerpt

Endodextranase was separated from dextranase inhibitor in culture filtrates of Streptococcus sobrinus by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) in gel slabs containing blue dextran. Sample preparation included dissociation of the enzyme from its inhibitor by boiling...

Topics

Dextranase
Electrophoresis, Polyacrylamide Gel
Enzyme Stability
Hydrogen-Ion Concentration
Molecular Weight
Mutation
Streptococcus sobrinus

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