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Article

Evaluation of the Polymerase Chain Reaction for Diagnosis of Lassa Virus Infection

1 Aug 1993

Abstract excerpt

We evaluated the polymerase chain reaction (PCR) and hybridization procedures for diagnosis of Lassa fever. Primers were derived from a region of the small RNA segment of Lassa virus coding for the glycoprotein. Serum samples stored for a 14-year period from patients in Sierra Leone, West Africa were examined retrospectively. Blinded samples were then tested prospectively. Eighty-eight virus isolation-negative...

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PMID: 8357084
DOI: 10.4269/ajtmh.1993.49.214

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