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Article

Nature and distribution of chromosomal intertwinings in Saccharomyces cerevisiae.

Molecular and cellular biology - 1 Feb 1994

Spell R M, Holm C

Abstract excerpt

To elucidate yeast chromosome structure and behavior, we examined the breakage of entangled chromosomes in DNA topoisomerase II mutants by hybridization to chromosomal DNA resolved by pulsed-field gel electrophoresis. Our study reveals that large and small chromosomes differ in the nature and distribution of their intertwinings. Probes to large chromosomes (450 kb or larger) detect chromosome breakage, but probes...

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Identifiers

PMID: 8289822
DOI: 10.1128/mcb.14.2.1465-1476.1994
PMCID: PMC358502

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