Article
Evidence for lysine 80 as general base catalyst of leucine dehydrogenase.
The Journal of biological chemistry - 25 Dec 1993
Sekimoto T, Matsuyama T, Fukui T, Tanizawa K
Abstract excerpt
To elucidate the functional role of the lysyl residue highly conserved in NAD(P)(+)-dependent amino acid dehydrogenases, Lys-80 of leucine dehydrogenase from Bacillus stearothermophilus has been mutated into Ala, Arg, or Gln. All of the mutant enzymes had markedly reduced activities in the oxidat...
Topics
- Amines
- Amino Acid Oxidoreductases
- Bacillus
- Base Sequence
- Catalysis
- DNA Primers
- Enzyme Activation
- Geobacillus stearothermophilus
- Hydrogen-Ion Concentration
- Keto Acids
- Leucine Dehydrogenase
- Lysine
- Molecular Sequence Data
- Mutation