Article
Mutation in GDP-fucose synthesis genes of Sinorhizobium fredii alters Nod factors and significantly decreases competitiveness to nodulate soybeans.
Molecular plant-microbe interactions : MPMI - 1 Mar 1999
Lamrabet Y, Bellogín R A, Cubo T, Espuny R, Gil A, Krishnan H B, Megias M, Ollero F J, Pueppke S G, Ruiz-Sainz J E, Spaink H P, Tejero-Mateo P, Thomas-Oates J, Vinardell J M
Abstract excerpt
We mutagenized Sinorhizobium fredii HH103-1 with Tn5-B20 and screened about 2,000 colonies for increased beta-galactosidase activity in the presence of the flavonoid naringenin. One mutant, designated SVQ287, produces lipochitooligosaccharide Nod factors (LCOs) that differ from those of the parental strain. The nonreducing N-acetylglucosamine residues of all of the LCOs of mutant SVQ287 lack fucose and...
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