Article
Amino acid residues required for the activity of avrD alleles.
Molecular plant-microbe interactions : MPMI - 1 Jan 2000
Yucel I, Keen N T
Abstract excerpt
Certain Pseudomonas syringae pathovars harbor avrD alleles belonging to two different homology classes. The nonfunctional avrD allele of P. s. pv. glycinea is highly homologous to active class II avrD alleles but has five unique amino acid substitutions. Three of these five amino acid changes were shown to be absolutely required for restoration of avrD activity to the P. s. pv. glycinea allele by oligonucleotide...
Topics
- Alleles
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutagenesis, Site-Directed
