Article
Preparation and characterization of a recombinant DNA-derived ovine growth hormone variant internally labelled with sulphur-35.
Journal of molecular endocrinology - 1 Dec 1993
Wallis M, Gwilliam D J, Wallis O C
Abstract excerpt
125I-Labelled polypeptide hormones have been extremely valuable for radioimmunoassays, receptor-binding studies and investigation of the processing and metabolism of hormones. However, such externally labelled material has the disadvantage that addition of one or more iodine atoms may alter the p...
Topics
- Animals
- Cloning, Molecular
- DNA, Recombinant
- Escherichia coli
- Female
- Genetic Variation
- Growth Hormone
- In Vitro Techniques
- Inclusion Bodies
- Microsomes, Liver
- Pregnancy
- Protein Conformation
- Rabbits
- Sheep
- Sulfur Radioisotopes
