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Article

Protonation and free energy changes associated with formation of QBH2 in native and Glu-L212-->Gln mutant reaction centers from Rhodobacter sphaeroides.

Biochemistry - 8 Feb 1994

McPherson P H, Schönfeld M, Paddock M L, Okamura M Y, Feher G

Abstract excerpt

Formation of the quinol QBH2 in Glu-L212-->Gln mutant [EQ(L212)] reaction centers (RCs) from Rhodobacter sphaeroides was investigated by measuring the proton uptake (using dyes), UV absorption changes, and free energy changes associated with the two-electron reduction of QB. The advantage of using the EQ(L212) RCs for these studies is that the individual protonation steps can be kinetically resolved and analyzed;...

Topics

Glutamine
Glycine
Hydrogen-Ion Concentration
Hydroquinones
Kinetics
Mutation
Photosynthetic Reaction Center Complex Proteins
Protons
Rhodobacter sphaeroides
Spectrophotometry, Ultraviolet

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Thermodynamics

Source

Identifiers

PMID: 8110749
DOI: 10.1021/bi00171a018

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8110749-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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