Article
Small weak acids stimulate proton transfer events in site-directed mutants of the two ionizable residues, GluL212 and AspL213, in the QB-binding site of Rhodobacter sphaeroides reaction center.
FEBS letters - 20 May 1991
Takahashi E, Wraight C A
Abstract excerpt
Mutations of the two ionizable residues, GluL212 and AspL213, in the secondary quinone (QB) binding site of reaction centers (RCs) from Rhodobacter sphaeroides cause major dysfunctions in the proton transfer processes leading to the formation of quinol. Mutant RCs with AspL213----Asn are especially severely blocked, and the rate of the proton-limited transfer of the second electron is at least 10(4) times slower...
Topics
- Aspartic Acid
- Electrons
- Glutamine
- Hydrogen-Ion Concentration
- Mutagenesis, Site-Directed
- Mutation
- Photosynthetic Reaction Center Complex Proteins
- Protons
- Rhodobacter sphaeroides