Excision and transposition of Tn5 upon insertion in the hha gene of Escherichia coli.

doi:10.1139/m94-095

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Article

Excision and transposition of Tn5 upon insertion in the hha gene of Escherichia coli.

Canadian journal of microbiology - 1 Jul 1994

Badenas C, Madrid C, Juárez A

Abstract excerpt

We report the occurrence of Tn5 secondary transpositions in Escherichia coli HB101 hha::Tn5(pANN202-312). Tn5-induced hha mutants (hemolysin overproducers) segregated at high frequency either hemolysin-negative clones or clones showing the parental hemolytic phenotype (reduced hemolysin productio...

Topics

Base Sequence
Blotting, Southern
DNA Probes
DNA Transposable Elements
Escherichia coli
Genes, Bacterial
Hemolysin Proteins
Molecular Sequence Data
Mutation

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