Isolation and characterization of temperature-sensitive mutants of cyanophage N-1.

Article

Isolation and characterization of temperature-sensitive mutants of cyanophage N-1.

Acta virologica - 1 Feb 1994

Sarma T A, Singh R

Abstract excerpt

Optimal conditions for the induction of temperature-sensitive (ts) mutants of cyanophage N-1 were established after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). A treatment with MNNG (400 micrograms/ml) for 2 hrs at pH 8.0 induced ts-mutants at a maximum frequency of 1.46 x 10(-3...

Topics

Bacteriophages
Cyanobacteria
Methylnitronitrosoguanidine
Mutagenesis
Mutation
Temperature

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