Use of randomly amplified polymorphic DNA amplified by polymerase chain reaction markers to estimate the number of Aedes aegypti families at oviposition sites in San Juan, Puerto Rico.

Abstract excerpt

We report the application of a molecular genetic technique to estimate the number of full-sibling families of Aedes aegypti contained in oviposition traps. Randomly amplified polymorphic DNA amplified by the polymerase chain reaction markers were used to estimate the numbers and sizes of families in traps at field locations in San Juan, Puerto Rico. Forty-nine presumptive loci were amplified with five primers in...

Topics

• Aedes
• Alleles
• Animals
• Cluster Analysis
• DNA
• Female
• Gene Frequency
• Insect Vectors
• Oviposition
• Polymerase Chain Reaction
• Polymorphism, Genetic