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Article

Microsatellite-based parentage analysis of Aedes aegypti (Diptera: Culicidae) using nonlethal DNA sampling.

Journal of medical entomology - 1 Jan 2012

Wong Jacklyn, Chu Yui Yin, Stoddard Steven T, Lee Yoosook, Morrison Amy C, Scott Thomas W

Abstract excerpt

To track Aedes aegypti (L.) egg-laying behavior in the field in Iquitos, Peru, we developed methods for 1) sampling DNA from live mosquitoes and 2) high through-put parentage analysis using microsatellite markers. We were able to amplify DNA extracted from a single hind leg, but not from the pupal exuvia. Removal of a leg from teneral females caused no significant changes in female behavioral or life history...

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Identifiers

PMID: 22308775
DOI: 10.1603/me11152
PMCID: PMC3312012

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