Article
Use of genetic polymorphisms detected by the random-amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) for differentiation and identification of Aedes aegypti subspecies and populations.
The American journal of tropical medicine and hygiene - 1 Dec 1992
Ballinger-Crabtree M E, Black W C, Miller B R
Abstract excerpt
Amplification of random regions of genomic DNA using 10-base primers in the random-amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to differentiate and identify mosquito populations based on genetic variation. Genomic DNA was extracted from individual mosquitoes from 11 geographic populations of Aedes aegypti and amplified in PCR reactions using single primers of arbitrary nucleotide...
Topics
- Aedes
- Animals
- Base Sequence
- Cluster Analysis
- DNA
- Discriminant Analysis
- Female
- Genetic Variation
- Insect Vectors
- Male
- Molecular Sequence Data