Article
Deletion variants of L-hydroxyisocaproate dehydrogenase. Probing substrate specificity.
European journal of biochemistry - 1 Aug 1994
Feil I K, Lerch H P, Schomburg D
Abstract excerpt
The substrate specificity and catalytic activity of the dinucleotide-dependent L-2-hydroxyisocaproate dehydrogenase from Lactobacillus confusus (L-HicDH) have been altered by modifying an enzyme region which is assumed to be involved in substrate recognition. The design of the variant enzymes was based on an amino acid alignment of the modified region with the functionally related L-lactate dehydrogenases. The...
Topics
- Alcohol Oxidoreductases
- Amino Acid Sequence
- Binding Sites
- DNA Mutational Analysis
- Genetic Variation
- L-Lactate Dehydrogenase
- Lactobacillus
- Molecular Sequence Data
- Sequence Deletion
- Sequence Homology, Amino Acid
- Structure-Activity Relationship