Article
Molecular characterization of attenuated vaccine strains of transmissible gastroenteritis virus.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Jan 1994
Register K B, Wesley R D
Abstract excerpt
Previous studies in our laboratory demonstrated that 2 attenuated strains of transmissible gastroenteritis virus (TGEV) contain deletions affecting messenger (m) RNAs 2, 3, or 4. In this report, we have compared mRNAs of four modified-live virus vaccines for TGEV with the virulent Miller PP3 isolate to determine whether any transcriptional patterns are shared among attenuated strains. Using northern blot...
Topics
- Blotting, Northern
- DNA, Complementary
- Genes, Viral
- Genetic Variation
- Genome, Viral
- Open Reading Frames
- Polymerase Chain Reaction
- RNA, Messenger
- RNA, Viral
- Transcription, Genetic