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Article

Survey on the distribution of the gene 4 alleles of human rotaviruses by polymerase chain reaction.

Epidemiology and infection - 1 Jun 1994

Wu H, Taniguchi K, Wakasugi F, Ukae S, Chiba S, Ohseto M, Hasegawa A, Urasawa T, Urasawa S

Abstract excerpt

The presence of six gene 4 alleles (or VP4 genotypes) in human rotaviruses has been recognized. Using 16 representative cultivable human rotavirus strains, we confirmed the specificity of VP4 genotyping by polymerase chain reaction (PCR) using the nested oligonucleotides specific to each of the four representative gene 4 alleles. Using the PCR, we surveyed the gene 4 alleles of 199 human rotaviruses in stools...

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Identifiers

PMID: 8005227
DOI: 10.1017/s0950268800051311
PMCID: PMC2271515

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