Article
Survey on the distribution of the gene 4 alleles of human rotaviruses by polymerase chain reaction.
Epidemiology and infection - 1 Jun 1994
Wu H, Taniguchi K, Wakasugi F, Ukae S, Chiba S, Ohseto M, Hasegawa A, Urasawa T, Urasawa S
Abstract excerpt
The presence of six gene 4 alleles (or VP4 genotypes) in human rotaviruses has been recognized. Using 16 representative cultivable human rotavirus strains, we confirmed the specificity of VP4 genotyping by polymerase chain reaction (PCR) using the nested oligonucleotides specific to each of the f...
Topics
- Alleles
- Base Sequence
- Capsid
- DNA Primers
- DNA, Viral
- Diarrhea
- Enzyme-Linked Immunosorbent Assay
- Feces
- Genotype
- Humans
- Japan
- Molecular Sequence Data
- Polymerase Chain Reaction
- Rotavirus
- Rotavirus Infections
- Sensitivity and Specificity
- Serotyping
- Thailand