Article
How is it that microsatellites and random oligonucleotides uncover DNA fingerprint patterns?
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Sept 1994
Kashi Y, Nave A, Darvasi A, Gruenbaum Y, Soller M, Beckmann J S
Abstract excerpt
Minisatellites, microsatellites, and short random oligonucleotides all uncover highly polymorphic DNA fingerprint patterns in Southern analysis of genomic DNA that has been digested with a restriction enzyme having a 4-bp specificity. The polymorphic nature of the fragments is attributed to tandem repeat number variation of embedded minisatellite sequences. This explains why DNA fingerprint fragments are...
Topics
- Animals
- Base Sequence
- Blotting, Southern
- Cattle
- DNA Fingerprinting
- DNA Primers
- DNA, Satellite
- Deoxyribonuclease BamHI
- Deoxyribonucleases, Type II Site-Specific
- Genetic Variation
