Generation of bovine multisite haplotypes using random cosmid clones.

Abstract excerpt

One hundred ten random cosmids were used to probe Southern blots of DNA from nine unrelated cattle digested with 12 restriction enzymes. Although only one-third of the expected fragments were explored, 85% of the cosmids revealed at least one polymorphism. The mean heterozygosity of the generated haplotypes was estimated at 51.9%. A surprisingly high proportion of polymorphisms (approximately 25%) was attributed...

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