Generation of bovine multisite haplotypes using random cosmid clones.

doi:10.1016/0888-7543(91)90177-g

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Article

Generation of bovine multisite haplotypes using random cosmid clones.

Genomics - 1 Aug 1991

Steele M R, Georges M

Abstract excerpt

One hundred ten random cosmids were used to probe Southern blots of DNA from nine unrelated cattle digested with 12 restriction enzymes. Although only one-third of the expected fragments were explored, 85% of the cosmids revealed at least one polymorphism. The mean heterozygosity of the generated...

Topics

Animals
Blotting, Southern
Cattle
Cloning, Molecular
Cosmids
DNA
DNA Probes
DNA Restriction Enzymes
Genetic Linkage
Genetic Variation
Haplotypes
Heterozygote
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length

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