Article
T-DNA-insert-independent mutations induced in transformed plant cells during Agrobacterium co-cultivation.
Transgenic research - 1 Sept 1994
Márton L, Hrouda M, Pécsváradi A, Czakó M
Abstract excerpt
Transformation frequencies were determined for 1n, 2n, and 4n Nicotiana plumbaginifolia protoplast cultures in Agrobacterium-mediated gene transfer experiments. An unexpected large drop (50%) in plating efficiencies was observed in the non-selected (control) 1n populations after transformation tr...
Topics
- Amino Acid Oxidoreductases
- Apoenzymes
- Cells, Cultured
- DNA, Bacterial
- Escherichia coli
- Gene Transfer Techniques
- Genetic Vectors
- Genotype
- Mutagenesis, Insertional
- Nitrate Reductases
- Plants, Genetically Modified
- Plants, Toxic
- Protoplasts
- Rhizobium
- Nicotiana
- Xanthine Dehydrogenase