Article
T-DNA-insert-independent mutations induced in transformed plant cells during Agrobacterium co-cultivation.
Transgenic research - 1 Sept 1994
Márton L, Hrouda M, Pécsváradi A, Czakó M
Abstract excerpt
Transformation frequencies were determined for 1n, 2n, and 4n Nicotiana plumbaginifolia protoplast cultures in Agrobacterium-mediated gene transfer experiments. An unexpected large drop (50%) in plating efficiencies was observed in the non-selected (control) 1n populations after transformation treatment with virulent strains. This effect was not observed in the 2n or 4n cultures or in the 1n cultures when treated...
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