Article
A dual-immunocytochemical method to localize c-fos protein in specific neurons based on their content of neuropeptides and connectivity.
Histochemistry - 1 Apr 1994
Mikkelsen J D, Larsen P J, Sørensen G G, Woldbye D, Bolwig T G, Hastings M H, Ebling F J
Abstract excerpt
Enhanced expression of the immediate early gene c-fos has been used as a marker of cellular activation in many different neuronal pathways. We wished to determine the neurochemical content and the connectivity of neurons, in which expression of c-fos is induced. For this purpose, a dual-immunocytochemical staining technique has been developed with avidin-biotin-peroxidase labelling using diaminobenzidine as the...
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