Validation studies on the forensic analysis of restriction fragment length polymorphism (RFLP) on LE agarose gels without ethidium bromide: effects of contaminants, sunlight, and the electrophoresis of varying quantities of deoxyribonucleic acid (DNA).

Article

Validation studies on the forensic analysis of restriction fragment length polymorphism (RFLP) on LE agarose gels without ethidium bromide: effects of contaminants, sunlight, and the electrophoresis of varying quantities of deoxyribonucleic acid (DNA).

Journal of forensic sciences - 1 May 1994

Laber T L, Giese S A, Iverson J T, Liberty J A

Abstract excerpt

This study was designed to analyze the effects of sunlight, various contaminants (those found typically in forensic samples) and the electrophoresis of varying quantities of DNA on the restriction fragment length polymorphism (RFLP) patterns produced from DNA isolated from blood and semen stains. The DNA RFLP patterns were obtained following Hae III restriction enzyme digestion, low electroendosmotic (LE) agarose...

Topics

Alleles
DNA
Electrophoresis, Agar Gel
Environmental Pollutants
Ethidium
Forensic Medicine
Humans
Male
Ointments
Polymorphism, Restriction Fragment Length

Reproducibility of Results

Semen

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