Article
Anther culture and Hordeum bulbosum-derived barley doubled haploids: mutations and methylation.
Molecular & general genetics : MGG - 1 Dec 1993
Devaux P, Kilian A, Kleinhofs A
Abstract excerpt
Anther culture and Hordeum bulbosum-derived doubled haploid (DH) lines of barley (Hordeum vulgare L.) were analyzed for RFLP and RAPD polymorphisms. Polymorphisms were not detected in the anther culture- or H. bulbosum-derived DH lines among 273 RFLP and 89 polymerase chain reaction (PCR)-amplified DNA fragments assayed. It was calculated that base substitution or small deletion/insertion mutations had not been...
Topics
- Base Sequence
- Cells, Cultured
- DNA Primers
- Haploidy
- Hordeum
- Methylation
- Molecular Sequence Data
- Mutation
- Ploidies
- Polymorphism, Restriction Fragment Length
- Seeds
