A rapid, simple, and reliable technique for preparation of antisera against idiotypes of homogeneous immunoglobulins.

Abstract excerpt

One-step immunization of guinea pigs with homogeneous immunoglobulins partly purified by micro-agar electrophoresis represents a rapid and reliable microtechnique for the preparation of antisera against the idiotype of the homogeneous immunoglobulins. Testing of these antisera by immunodiffusion techniques requires agar plates containing 3% polyethyleneglycol 6000.

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