A rapid, simple, and reliable technique for preparation of antisera against idiotypes of homogeneous immunoglobulins.

doi:10.1111/j.1423-0410.1978.tb02894.x

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Article

A rapid, simple, and reliable technique for preparation of antisera against idiotypes of homogeneous immunoglobulins.

Vox sanguinis - 1 Jan 2000

Radl J, de Glopper E, de Groot G

Abstract excerpt

One-step immunization of guinea pigs with homogeneous immunoglobulins partly purified by micro-agar electrophoresis represents a rapid and reliable microtechnique for the preparation of antisera against the idiotype of the homogeneous immunoglobulins. Testing of these antisera by immunodiffusion techniques requires agar plates containing 3% polyethyleneglycol 6000.

Topics

Animals
Electrophoresis, Agar Gel
Epitopes
Genotype
Guinea Pigs
Immune Sera
Immunization
Immunoglobulins
Mice
Polyethylene Glycols
Rabbits
Time Factors

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