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Article

Assessing the viability of mutant and manipulated sperm by artificial insemination of Caenorhabditis elegans.

Genetics - 1 Nov 1994

LaMunyon C W, Ward S

Abstract excerpt

We describe a protocol for artificial insemination of Caenorhabditis elegans which we used to evaluate the viability of sperm from different strains and of sperm activated in vitro. Worms can be artificially inseminated with almost 100% success. Both male and hermaphrodite sperm can be used for insemination. Sperm from a sterile hermaphrodite [fem-3(q23ts)] were found to be viable. As with normal mating, male...

Topics

Animals
Caenorhabditis elegans
Cell Survival
Disorders of Sex Development
Female
Insemination, Artificial
Male
Mutation
Oocytes
Spermatozoa

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 7851766
DOI: 10.1093/genetics/138.3.689
PMCID: PMC1206219

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7851766-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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