Article
Molecular cloning of Rab-related genes in the yeast Yarrowia lipolytica. Analysis of RYL1, an essential gene encoding a SEC4 homologue.
Current genetics - 1 Jan 1995
Pertuiset B, Beckerich J M, Gaillardin C
Abstract excerpt
Small GTP-binding proteins of the Rab family are involved in the vesicular traffic inside eukaryotic cells. A gene library from the yeast Yarrowia lipolytica was screened with an oligonucleotide deduced from a highly conserved sequence in the Rab family. Four different genes were isolated. One of...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Conserved Sequence
- Fungal Proteins
- GTP-Binding Proteins
- Gene Deletion
- Genetic Vectors
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Saccharomycetales
- Sequence Alignment
- rab GTP-Binding Proteins