Article
Molecular characterization of glycophorin A transcripts in human erythroid cells using RT-PCR, allele-specific restriction, and sequencing.
Vox sanguinis - 1 Jan 1995
DuPont B R, Grant S G, Oto S H, Bigbee W L, Jensen R H, Langlois R G
Abstract excerpt
Glycophorin A (GPA) is an erythroid-lineage-specific membrane sialoglycoprotein which occurs in two allelic forms, M and N, which form the antigens of the MN blood group. Purified cDNAs and RNAs isolated from peripheral blood and erythroleukemia cell lines, HEL and K562, were used to develop an RT-PCR technique for amplifying GPA gene transcripts (GYPA). The relative expression of transcripts from the M and N...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Erythroid Precursor Cells
- Glycophorins
- Humans
- Leukemia, Erythroblastic, Acute
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length