Mutations in the complementarity-determining regions do not cause differences in free energy during the process of formation of the activated complex between an antibody and the corresponding protein antigen.

Abstract excerpt

Effects of mutations on antigen-antibody interactions were energetically examined using several kinds of Fv fragments, derivatives of a monoclonal antibody, D1.3, that is specific for hen egg-white lysozyme. According to the transition-state theory, the thermodynamic parameters involved in pre and post-processes of formation of the activated complex were estimated separately. In the pre-process, there is a strong...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Antigen-Antibody Reactions
• Share to Chickens