Enhanced expression of IL‐8 in normal human keratinocytes and human keratinocyte cell line HaCaT <i>in vitro</i> after stimulation with contact sensitizers., tolerogens and irritants

Abstract excerpt

To investigate the interleukin-8 production of keratinocytes after stimulation in vitro we have used various agents: (i) contact sensitizer (2,4-dinitrofluorobenzene, 3-n-pentadecylcatechol); (ii) tolerogen (5-methyl-3-n-pentadecylcatechol); (iii) irritant (sodium lauryl sulfate). Interleukin-8 gene expression was assessed by northern blot hybridization of the total cytoplasmic RNA extracted from subconfluent...

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